of cell carcinomas (OSCCs). However, in this study

 of ?-catenin. These
results propose that deletions occurring in FAT1 gene contributes to cancerous
dispersed morphology. settlementNishikawa et al. analyzed the expression levels of mRNAs of FAT1, which
exist in human OSCC cell lines with RT-PCR. The levels showed diverse results
for all cultured cell lines except SAS and SCC66. Additionally, immunofluorescence
was conducted with the same aim. However, alike result got yield. Results got
from double immunofluorescence done with 3 FAT1-positive and 1 FAT1-negative
cell lines showed that ?-catenin is localized in the cell membrane of
FAT1-expressing OSCCs, however in the FAT1-negative cell since the gene is
absent, ?-catenin’s got present only in cytoplasm and nuclei due to cell to
cell adhesion being lack, hence have a undifferentiated shape of cells. In the
light of points mentioned before; the gene FAT1 can be considered to be as
tumor suppressor. Additionally, the gene can regulate cell proliferation in an
indirect way. Expression of gene is inversely related to proliferation in vascular
muscle cells When the expression of gene is restricted, proliferation in
vascular muscle cells gets increased; when the expression is promoted, the cell
proliferation got decreased. Thus, it was predicted that suppression of the
gene would also raise the proliferation of oral squamous cell carcinomas (OSCCs).
 However, in this study when the mRNAs of
FAT1 genes were silenced, circumscribed effects were observed on the
proliferation of OSCC cell lines. Therefore, it can be concluded that FAT cadherin
has a more minor role for cell proliferation of OSCC when it is compared with
other tumor suppressor genes. Occurrence of deletion in the product of the gene
FAT1 might have a major role in the metamorphosis of various characteristics of
carcinoma cells including; decrement of cell to cell adhesion, morphology and
cell polarity. One of the characteristic roles of gene FAT1 products is to
maintain morphology and polarity by collaborating with the stress fibers
?-catenin and Vasp/Ena which binds with the cytoplasmic domain of the gene.
Furthermore, the gene contains a nuclear localization signal sequence and by
translocating its cytoplasmic domain into the nucleus, cell polarity and
migration cell polarity and migration can occur as result. This study also
revealed that, when the gene FAT1 was silenced a significant reduction of cell
to cell adhesion occurred alongside alteration in cell morphology due to the
fact dispersed